Materials
| Name | Company | Catalog Number | Comments |
| Dextran sulfate | Fisher | BP1585-100 | |
| Chitosan, low molecular weight | Sigma | 448869 | |
| Zinc sulfate heptahydrate | Sigma | 204986 | |
| D-Mannitol | Sigma | M9546 | |
| UltraPure water | Invitrogen | 10977-023 | |
| SDF-1α | Prepared according to reference 8. | ||
| Syringe filter, PES membrane 0.22 μm | Millipore | SLGP033RS | |
| Magnetic Micro Stirring Bars (2 x 7 mm) | Fisher | 14-513-63 | |
| Glass vial Kit; SUN-SRi | Fisher | 14-823-182 | |
| Delsa Nano C Particle Analyzer | Backman Coulter | ||
| Eppendorf UVette Cuvets | Eppendorf | 952010069 | |
| 4–20% Mini-PROTEAN TGX Gel | Bio-Rad | 456-1096 | |
| GelCode Blue Safe Protein Stain | Fisher | PI-24592 | |
| Molecular Imager VersaDoc MP 4000 System | BioRad | 170-8640 | |
| Corning Transwell Permeable Supports | Corning | 3421 | |
| Accuri C6 Flow Cytometer | BD Biosciences | ||
| Dulbecco’s phosphate buffered saline | Sigma | D8537 | |
| Pyrogent plus kit | Fisher | NC9753738 |
References
- Delair, T. Colloidal polyelectrolyte complexes of chitosan and dextran sulfate towards versatile nanocarriers of bioactive molecules. Eur J Pharm Biopharm. 78 (1), 10-18 (2011).
- Morachis, J. M., Mahmoud, E. A., Almutairi, A. Physical and chemical strategies for therapeutic delivery by using polymeric nanoparticles. Pharmacol Rev. 64 (3), 505-519 (2012).
- Yue, Z. G., et al. Surface charge affects cellular uptake and intracellular trafficking of chitosan-based nanoparticles. Biomacromolecules. 12 (7), 2440-2446 (2011).
- Ghadge, S. K., Muhlstedt, S., Ozcelik, C., Bader, M. SDF-1alpha as a therapeutic stem cell homing factor in myocardial infarction. Pharmacol Ther. 129 (1), 97-108 (2011).
- Sharma, M., Afrin, F., Satija, N., Tripathi, R. P., Gangenahalli, G. U. Stromal-derived factor-1/CXCR4 signaling: indispensable role in homing and engraftment of hematopoietic stem cells in bone marrow. Stem Cells Dev. 20 (6), 933-946 (2011).
- Sadir, R., Baleux, F., Grosdidier, A., Imberty, A., Lortat-Jacob, H. Characterization of the stromal cell-derived factor-1alpha-heparin complex. J Biol Chem. 276 (11), 8288-8296 (2001).
- Amara, A., et al. Stromal cell-derived factor-1alpha associates with heparan sulfates through the first beta-strand of the chemokine. J Biol Chem. 274 (34), 23916-23925 (1999).
- Sadir, R., Imberty, A., Baleux, F., Lortat-Jacob, H. Heparan sulfate/heparin oligosaccharides protect stromal cell-derived factor-1 (SDF-1)/CXCL12 against proteolysis induced by CD26/dipeptidyl peptidase IV. J Biol Chem. 279 (42), 43854-43860 (1074).
- Yin, T., et al. SDF-1alpha in glycan nanoparticles exhibits full activity and reduces pulmonary hypertension in rats. Biomacromolecules. 14 (11), 4009-4020 (2013).
- Huang, M., Vitharana, S. N., Peek, L. J., Coop, T., Berkland, C. Polyelectrolyte complexes stabilize and controllably release vascular endothelial growth factor. Biomacromolecules. 8 (5), 1607-1614 (2007).
- McCall, R. L., Sirianni, R. W. PLGA nanoparticles formed by single- or double-emulsion with vitamin E-TPGS. J Vis Exp. (82), 51015 (2013).
- Carrillo-Conde, B. R., Roychoudhury, R., Chavez-Santoscoy, A. V., Narasimhan, B., Pohl, N. L. High-throughput synthesis of carbohydrates and functionalization of polyanhydride nanoparticles. J Vis Exp. (65), 3967 (2012).
- Xu, J., Amiji, M. Therapeutic gene delivery and transfection in human pancreatic cancer cells using epidermal growth factor receptor-targeted gelatin nanoparticles. J Vis Exp. (59), e3612 (2012).
- Lauten, E. H., et al. Nanoglycan complex formulation extends VEGF retention time in the lung. Biomacromolecules. 11 (7), 1863-1872 (2010).
- Schatz, C., Domard, A., Viton, C., Pichot, C., Delair, T. Versatile and efficient formation of colloids of biopolymer-based polyelectrolyte complexes. Biomacromolecules. 5 (5), 1882-1892 (2004).
