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In Situ Ca 2+ billeddannelse af enteriske nervesystem

Published: January 29, 2015 doi: 10.3791/52506


Name Company Catalog Number Comments
BubbleStop Syringe Heater  AutoMate Scientific 10-4-35-G
CaCl2 Sigma C3306
Collagenase, Type II, powder Gibco 17101-015
Dispase Sigma-Aldrich 42613-33-2
Dissection tools Roboz 
DMSO Sigma-Aldrich D5879
Fixed-stage microscope Olympus  BX51WI
Fluo-4 AM dye Invitrogen F-14201
Glucose Sigma G8270
Insect pins Fine Science Tools Minutien Pins
iQ Live Cell Imaging Software Andor Andor iQ3
KCl Sigma P3911
MgCl2 Sigma M9272
NaCl Sigma S9888
NaH2PO4 Sigma S8282
NaHCO3 Sigma S6014
Neo sCMOS camera Andor Neo 5.5 sCMOS
Nicardipine Sigma N7510
Perfusion chamber Custom
Peristaltic pump Harvard Apparatus Model 720
Pluronic F-127  Invitrogen P3000MP
Probenecid Molecular Probes P36400
Scopolamine Sigma S1013 
Sutter Lambda DG-4 Sutter DG-4
Sylgard  Dow Corning 184
Temperature Controller Warner Instruments TC-344C



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<em>In Situ</em> Ca <sup>2+</sup> billeddannelse af enteriske nervesystem
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Fried, D. E., Gulbransen, B. D. In Situ Ca2+ Imaging of the Enteric Nervous System. J. Vis. Exp. (95), e52506, doi:10.3791/52506 (2015).More

Fried, D. E., Gulbransen, B. D. In Situ Ca2+ Imaging of the Enteric Nervous System. J. Vis. Exp. (95), e52506, doi:10.3791/52506 (2015).

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