Materials
Name | Company | Catalog Number | Comments |
Polyethylene imine | Polysciences | 23966-2 | |
DMEM medium | Lonza | BE12-614F | |
Fetal calf serum | Lonza | DE14-801F | |
Penicillin-streptomycin | Invitrogen | 15140-122 | |
Glutamin | Invitrogen | 25030-024 | |
matrigel | BD | BD 356231 | |
Advanced DMEM-F12 | Gibco | 12634-010 | |
N2 | Invitrogen | 17502-048 | |
B27 | Invitrogen | 17504-044 | |
N-acetyl cysteine | Sigma | A9165-1G | |
mouse Egf | Invitrogen | PMG8045 | |
Hepes 1 M | Invitrogen | 15630-056 | |
glutamax 100x | Invitrogen | 35050-038 | |
Chir 99021 | Axon | 1386 | |
Y27632 | Sigma | Y0503-5MG | |
polybrene | Sigma | 107689 | |
nicotinamide | Sigma | N0636 | |
Trypsin | Lonza | BE02-007E | |
puromycin | Sigma | P 7255 | |
Rneasy mini kit | Qiagen | 74106 | |
β-mercaptoethanol | Merck | 8,057,400,250 | |
Ovation Pico WTA system | NuGen | 3300-12 | |
paraformaldehyde | Sigma | 252549-1L | |
glass vial conical 12 mm x 75 mm 5 ml | VWR | LSUKM12 | |
Eosin Yellowish | VWR | 1,159,350,025 |
References
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