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Immunology and Infection

Анализируя функции тучных клеток doi: 10.3791/52753 Published: May 27, 2015

Materials

Name Company Catalog Number Comments
1% Antibiotic-Antimycotic Solution Corning cellgro 30-004-Cl
3 ml Syringe Falcon 309656
35 mm x 10 mm Dish Corning cellgro 430588
5 ml Polystyrene Round Bottom Tube Falcon 352058
Acetic Acid Glacial Fisher Scientific A35-500
Alcian Blue 8GX Rowley Biochemical Danver 33864-99-2
Allegra 6R Centrifuge Beckman
Anti-mouse CD16/32 (clone 93) Purified eBioscience 14-0161-81
2-Mercaptoethanol Sigma Aldrich M7522
BD 1 ml TB Syringe BD Syringe 309659
BD 22 G x 1 (0.7 mm x 25 mm) Needles BD Precision Glide Needle 205155
BD 25 G 5/8 Needles BD Syringe 305122
BD 30 G x 1/2 Needles BD Precision Glide 305106
Blue MAX Jr, 15 ml Polypropylene Conical Tube Falcon 352097
Chloroform Fisher Scientific C298-500
Cytoseal 60 Mounting Medium Richard-Allan Scientific 8310-4
Cytospin3 Shandon NA
DakoCytomation pen Dako S2002
Dulbecco Modified Eagle Medium (DMEM) 1x Corning cellgro 15-013-CM
Ethanol Sigma Aldrich E 7023-500ml
Fetal Bovine Serum Heat Inactivated Sigma Aldrich F4135-500ml
FITC Conjugated IgG2b K Rat Isotype Control eBioscience 14-4031-82
Fluorescein Isotiocyanate (FITC) Conjugated Anti-mouse KIT (CD117; clone 2B8) eBioscience 11-1171-82
Formaldehyde Fisher Scientific F79-500
Giemsa Stain Modified Sigma Aldrich GS-1L
Isothesia Henry Schein Animal Health 29405
May-Grunwald Stain Sigma Aldrich MG-1L
Multiwell 6 well plates Falcon 35 3046
Olympus BX60 Microscope Olympus NA
Paraplast Plus Tissue Embedding Medium Fisher Brand 23-021-400
PE Conjugated IgG Armenian Hamster Isotype Control eBioscience 12-4888-81
Phosphate-Buffered-Saline (PBS) 1x Corning cellgro 21-040-CV
Phycoerythrin (PE) Conjugated Anti-mouse FceRIa (clone MAR-1) eBioscience 12-5898-82
Propidium Iodide Staining Solution eBioscience 00-6990-50
Recombinant Mouse IL-3 Peprotech 213-13
Safranin-o Certified Sigma Aldrich S8884
Tissue culture flasks T25 25 cm2 Beckton Dickinson 353109
Tissue culture flasks T75 75 cm2 Beckton Dickinson 353110
Toluidine Blue 1% Aqueous LabChem-Inc LC26165-2
Recombinant Mouse SCF Peprotech 250-03

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References

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Анализируя функции тучных клеток<em&gt; В Vivo</em&gt; Использование &#39;<em&gt; Тучных клеток Knock-в</em&gt; &#39;Мыши
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Cite this Article

Gaudenzio, N., Sibilano, R., Starkl, P., Tsai, M., Galli, S. J., Reber, L. L. Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice. J. Vis. Exp. (99), e52753, doi:10.3791/52753 (2015).More

Gaudenzio, N., Sibilano, R., Starkl, P., Tsai, M., Galli, S. J., Reber, L. L. Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice. J. Vis. Exp. (99), e52753, doi:10.3791/52753 (2015).

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