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Immunology and Infection

Murine הינד לימב ארוך עצם Dissection ובידוד מח העצם

doi: 10.3791/53936 Published: April 14, 2016

Materials

Name Company Catalog Number Comments
Pinboard
Pins
70% Ethanol
Dissection scissors 
Dissection forceps
Kimwipes Kimberly-Clark 34120
16 G needle
1.5 ml Microcentrifuge tube
0.5 ml Microcentrifuge tube
Microcentrifuge

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References

  1. McHayleh, W. M., Ellerman, J., Roodman, G. D. Ch. 80. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Socieyt for Bone and Mineral Research. 379-381 (2008).
  2. Weilbaecher, K. N., Guise, T. A., McCauley, L. K. Cancer to bone: a fatal attraction. Nat Rev Cancer. 11, (6), 411-425 (2011).
  3. Pedersen, E. A., Shiozawa, Y., Pienta, K. J., Taichman, R. S. The prostate cancer bone marrow niche: more than just 'fertile soil. Asian J Androl. 14, (3), 423-427 (2012).
  4. Amend, S. R., et al. Thrombospondin-1 regulates bone homeostasis through effects on bone matrix integrity and nitric oxide signaling in osteoclasts. J Bone Miner Res. 30, (1), 106-115 (2015).
  5. Hurchla, M. A., et al. The epoxyketone-based proteasome inhibitors carfilzomib and orally bioavailable oprozomib have anti-resorptive and bone-anabolic activity in addition to anti-myeloma effects. Leukemia. 27, (2), 430-440 (2013).
  6. Rauch, D. A., et al. The ARF tumor suppressor regulates bone remodeling and osteosarcoma development in mice. PLoS One. 5, (12), e15755 (2010).
  7. Su, X., et al. The ADP receptor P2RY12 regulates osteoclast function and pathologic bone remodeling. J Clin Invest. 122, (10), 3579-3592 (2012).
  8. Dempster, D. W., et al. Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. 28, (1), 2-17 (2013).
  9. Begley, C. G., Ellis, L. M. Drug development: Raise standards for preclinical cancer research. Nature. 483, (7391), 531-533 (2012).
  10. Festing, M. F., Altman, D. G. Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J. 43, (4), 244-258 (2002).
Murine הינד לימב ארוך עצם Dissection ובידוד מח העצם
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Cite this Article

Amend, S. R., Valkenburg, K. C., Pienta, K. J. Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation. J. Vis. Exp. (110), e53936, doi:10.3791/53936 (2016).More

Amend, S. R., Valkenburg, K. C., Pienta, K. J. Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation. J. Vis. Exp. (110), e53936, doi:10.3791/53936 (2016).

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