Materials
Name | Company | Catalog Number | Comments |
Polydimethylsiloxane (PDMS) [Sylgard 184 Elastomer Kit] | Fisher Scientific (Ellsworth Adhesives) | NC0162601 | Reagents |
Polyethylene glycol dimethacrylate (PEGDM) | Prepared according to Lin-Gibson with PEG from Sigma Aldrich | 95904 | Reagents |
3-(Trimethoxysilyl)propyl methacrylate | Sigma Aldrich | M6514 | Reagents |
3-glycidoxypropyltrimethoxysilane | Sigma Aldrich | 440167 | Reagents |
Dimethyl Phenylphosphonite | Sigma Aldrich | 149470-5g | Reagents |
2,4,6-trimethylbenzoyl Chloride | Fisher Scientific | AC244280100 | Reagents |
2-Butanone | Fisher Scientific | AC149670010 | Reagents |
Lithium Bromide | Fisher Scientific | AC199871000 | Reagents |
Isopropanol | Sigma Aldrich | 190764 | Reagents |
Sigmacote (siliconizing reagent) | Sigma Aldrich | SL2 | Reagents |
Toluene | Fisher Scientific | AC36441-0010 | Reagents |
Phosphate Buffered Saline (PBS) | Hyclone | SH3025601 | Reagents |
Opti-MEM Reduced Serum Medium, no phenol red (phenol- and serum-free medium) | Life Technologies | 11058-021 | Reagents |
FuGENE HD Transfection Reagent (transfection reagent) | Promega | E2311 | Reagents |
Cellstripper (cell dissociation solution) | Corning | 25-056-CI | Reagents |
Growth Factor Reduced Matrigel, Phenol Free | Corning | 356231 | Reagents |
Forskolin | Sigma Aldrich | F6886 | Reagents |
Pipette Tips (10,200,1,000 μl) | Fisher Scientific | 02-707-474, 02-707-478, 02-707-480 | Disposable lab equipment |
Powder Free Examination Gloves | Fisher Scientific | 19-149-863B | Disposable lab equipment |
Aluminum foil | Fisher Scientific | 01-213-100 | Disposable lab equipment |
Biopsy punch (3 mm) | Miltex | 3332 | Disposable lab equipment |
Glass coverslips | Fisher Scientific | 12-544C | Disposable lab equipment |
Precoated glass coverslips (epoxysilane) | Arrayit | CCSL | Disposable lab equipment |
Glass slide | Fisher Scientific | 12550D | Disposable lab equipment |
Sterile vials (15 ml, 50 ml conical) | Fisher Scientific | 14-959-70C, 14-959-49A | Disposable lab equipment |
Cell culture dish (6-well) | Falcon | 351146 | Disposable lab equipment |
Epifluorescent Microscope with motorized stage | Nikon | MEA53100 (Eclipse TiE Inverted) | Large/non-disposable lab equipment |
Adjustable Specimen Holder for XY Stage | Nikon | 77011393 | Non-disposable lab equipment |
60X (1.3 NA) objective (CFI Plan Apochromat 60XH Lamda) | Nikon | MRD01605 | Non-disposable lab equipment |
CFP/YFP light source and excitation / emission filter holders (Lambda light source & wheel, Lambda emission filter system) | Nikon (Sutter Instrument reseller) | 77016231, 77016088 | Non-disposable lab equipment |
CYF/YFP filter set | Nikon (Chroma Technology Corp) | 77014928 | Non-disposable lab equipment |
Camera (ORCA Flash 4.0 v1) | Nikon (Hamamatsu reseller) | 77054076 | Non-disposable lab-equipment |
NIS-Elements 40.20.02 microscope software) | Nikon | MQS31100 | Non-disposable lab equipment |
Prism (spreadsheet and graphing software) | GraphPad Software, Inc | Version 6 | Non-disposable lab equipment |
OmniCure S1000 UV Spot Curing Lamp System with 365nm filter or other light source (λ = 365 or 405) | OmniCure | S1000 | Large/non-disposable lab equipment |
Pipette Aid | Drummond Scientific Co. | DP-100 | Non-disposable lab equipment |
Hemacytometer | Hausser Scientific | Reichert Bright-Line 1475 | Non-disposable lab equipment |
Vacuum desiccator chamber/degasser | Any | Large/non-disposable lab equipment | |
Tissue Culture Hood | Any | Large/non-disposable lab equipment | |
Chemical Fume Hood | Any | Large/non-disposable lab equipment | |
Oven | Any | Large/non-disposable lab equipment | |
Spatula | Any | Non-disposable lab equipment | |
Beaker | Any | Non-disposable lab equipment | |
Incubator | Any | Large/non-disposable lab equipment |
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