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June 2015: This Month in JoVE - Celebrating JoVE's 100th Issue

Published: June 2, 2015 doi: 10.3791/5730
1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production


Practical Methodology of Cognitive Tasks Within a Navigational Assessment

Manon Robillard1, Chantal Mayer-Crittenden1, Annie Roy-Charland2, Michèle Minor-Corriveau1, Roxanne Bélanger1

1Speech-Language Pathology Program, Laurentian University, 2Psychology Department, Laurentian University

Children who have complex communication needs can benefit from the use of a speech-generating device (SGD). Cognitive skills were identified as having an impact on the ability to navigate between levels of SGDs. This protocol describes the steps needed for Speech-language Pathologists to assess cognitive skills and navigational abilities.

Quantifying Learning in Young Infants: Tracking Leg Actions During a Discovery-learning Task

Barbara Sargent1, Hendrik Reimann2, Masayoshi Kubo3, Linda Fetters1

1Division of Biokinesiology & Physical Therapy at the Herman Ostrow School of Dentistry, University of Southern California, 2Department of Kinesiology, Temple University, 3Department of Physical Therapy, Niigata University of Health and Welfare

A method is described in which 3-4 month old infants learn a task by discovery and their leg movements are captured to quantify the learning process.

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

M. Yakut Ali1, Sandeep V. Anand1, Krishnarao Tangella2,3, Davendra Ramkumar2,3, Taher A. Saif1,4

1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 2College of Medicine, University of Illinois at Urbana-Champaign, 3Provena Covenant Medical Centre, 4Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign

A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Viviana Meraviglia*1, Alessandra Zanon*1, Alexandros A. Lavdas1, Christine Schwienbacher1, Rosamaria Silipigni2, Marina Di Segni2, Huei-Sheng Vincent Chen3, Peter P. Pramstaller1, Andrew A. Hicks1, Alessandra Rossini1

1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute

Induced pluripotent stem cells (iPSCs) represent a source of patient-specific tissues for clinical applications and basic research. Here, we present a detailed protocol to reprogram human peripheral blood mononuclear cells (PBMNCs) obtained from frozen buffy coats into viral-free iPSCs using non-integrating episomal plasmids.


No conflicts of interest declared.

June 2015: This Month in JoVE - Celebrating JoVE's 100th Issue
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Chao, W., Kolski-Andreaco, A. June 2015: This Month in JoVE - Celebrating JoVE's 100th Issue. J. Vis. Exp. (100), e5730, doi:10.3791/5730 (2015).More

Chao, W., Kolski-Andreaco, A. June 2015: This Month in JoVE - Celebrating JoVE's 100th Issue. J. Vis. Exp. (100), e5730, doi:10.3791/5730 (2015).

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