JoVE Monthly Highlights: May 2018

1JoVE Content Production

Tickling, a Technique for Inducing Positive Affect When Handling Rats

Sylvie Cloutier1,2, Megan R. LaFollette3, Brianna N. Gaskill3, Jaak Panksepp1, Ruth C. Newberry4

This article demonstrates the standardized application of playful handling, a tickling technique designed to mimic rat rough-and-tumble play. This technique is effective at reducing fearful reactions to humans and generating positive affect when rats are handled for common husbandry activities and medical and research procedures such as injection.

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Amanda F. Bolgioni1, Marc A. Vittoria1, Neil J. Ganem1,2

Live-cell imaging provides a wealth of information on single cells or whole populations that is unattainable by fixed cell imaging alone. Here, live-cell imaging protocols to assess cell fate decisions following treatment with the anti-mitotic drug paclitaxel are described.

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Behnom Farboud*1,2, Erin Jarvis*1, Theodore L. Roth*3,4,5,6, Jiyung Shin*1,3, Jacob E. Corn1,3, Alexander Marson3,5,6,7,8,9, Barbara J. Meyer1,2, Nipam H. Patel1,10, Megan L. Hochstrasser3
* These authors contributed equally

Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model organisms.

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Robert W Epps1, Kobi C Felton1, Connor W Coley2, Milad Abolhasani1

Detailed herein are the operation and assembly protocols of a modular microfluidic screening platform for the systematic characterization of colloidal semiconductor nanocrystal syntheses. Through fully adjustable system arrangements, highly efficient spectra collection may be carried out across 4 orders of magnitude reaction time scales within a mass transfer-controlled sampling space.