Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Biology

拆装时间推移显微镜监测肌动蛋白

doi: 10.3791/66 Published: November 8, 2006

Summary

Protocol

or Start trial to access full content. Learn more about your institution’s access to JoVE content here
  1. 构造一个在视频流室。确保封口膜密封紧,用洗净的盖玻片。
  2. 孵育肌动蛋白结合剂5-10“室。
  3. 座具有阻止蛋白质的非特异性结合位点上的玻璃盖玻片。
  4. 肌动蛋白聚合箱内流淌在G -肌动蛋白聚合缓冲区。
  5. 冲蚀unpolymerized肌动蛋白在过剩缓冲区流动。

Subscription Required. Please recommend JoVE to your librarian.

拆装时间推移显微镜监测肌动蛋白
Play Video
PDF DOI

Cite this Article

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).More

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

Less
Copy Citation Download Citation Reprints and Permissions
View Video

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter