acupuncture-treatment-mouse-model-chronic-hypoxia-induced-cognitive

Acupuncture Treatment in a Mouse Model of Chronic Hypoxia-Induced Cognitive Dysfunction

The treatment of central nervous disorders has consistently posed significant challenges to the medical field. Acupuncture, a non-pharmacological practice ...

cardio-cerebral-protection-electroacupuncture-murine-model-chronic

Cardio-Cerebral Protection by Electroacupuncture in a Murine Model of Chronic Hypoxia

This study established a 12-week chronic hypoxia murine model to investigate the cardio-cerebral protective effects of electroacupuncture. Existing ...

the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Scalp acupuncture is a contemporary acupuncture method based on the fundamental theories of traditional acupuncture, which has been widely used in patients with stroke in China. However, the effectiveness is controversial due to lack of solid experimental evidence.

Scalp Electroacupuncture Promotes Angiogenesis after Stroke in Rats by Activation of Wnt/-Catenin Signal Pathway.

This study aimed to investigate the possible effects and underlying mechanisms of Kangzhi syrup on ovalbumin- (OVA-) induced cough variant asthma (CVA) in guinea pigs.

Therapeutic Effects of Kangzhi Syrup in a Guinea Pig Model of Ovalbumin-Induced Cough Variant Asthma.

This study aimed to investigate the mechanistic action and therapeutic effects of Bufei decoction on idiopathic pulmonary fibrosis (IPF) after inhalation of bleomycin.

Bufei Decoction Alleviated Bleomycin-Induced Idiopathic Pulmonary Fibrosis in Mice by Anti-Inflammation.

The paper introduces the clinical experience of professor  in treatment of  (orifice obstruction) with acupuncture. In pathogenesis,  refers to the obstruction of  movement and abnormal ascending and descending of . "Obstruction of  orifices" is the  (symptoms), while "obstruction of brain orifice" is  (root cause) of diseases. In the principle of treatment,  and  should be considered simultaneously, and it is proposed to regulating the spirit and unblocking the orifices in treatment. Baihui (GV 20) and the cognitive emotional area on the head are selected for regulating the spirit and opening the brain orifice, focusing on the root cause of diseases. Based on the connection between the symptoms and the cerebral cortical functions, the different points on the head are used, the  points (Hegu [LI 4] and Taichong [LR 3]) and -source points combined, and the acupoint prescription is composed according to the interior-exterior relationship of meridians. With repetitive transcranial acupuncture stimulation at the points on the head, the therapeutic effect is strengthened. It provides a novel approach to treatment of diseases related to .

Professor 's clinical experience in treatment of  with acupuncture.

This study aims to explore the neuroprotective effects of scalp Electroacupuncture (EA) on ischemic stroke, with a specific focus on the role of electrical stimulation (ES). Employing a rat model of middle cerebral artery occlusion (MCAO), we used methods such as Triphenyl tetrazolium chloride staining, micro-CT scanning, Enzyme linked immunosorbent assay (ELISA), and immunofluorescence to assess the impacts of EA. We further conducted RNA-seq analysis and in vitro experiments with organotypic brain slices and cerebral organoids to explore the underlying mechanisms. Our research revealed that EA notably reduced cerebral infarct volume and improved regional cerebral blood flow in rats following MCAO. Micro-CT imaging showed improved vascular integrity in EA-treated groups. Histological analyses, including HE staining, indicated reduced brain tissue damage. ELISA demonstrated a decrease in pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, suggesting improved blood-brain barrier function. Immunofluorescence and Western blot analyses revealed that EA treatment significantly inhibited microglial and astrocytic overactivation. RNA-seq analysis of brain tissues highlighted a downregulation of immune pathways and inflammatory responses, confirming the neuroprotective role of EA. This was further corroborated by in vitro experiments using organotypic brain slices and cerebral organoids, which showcased the efficacy of electrical stimulation in reducing neuroinflammation and protecting neuronal cells. The study highlights the potential of scalp EA, particularly its ES component, in treating ischemic stroke. It provides new insights into the mechanisms of EA, emphasizing its efficacy in neuroprotection and modulation of neuroinflammation, and suggests avenues for optimized treatment strategies in stroke therapy.

Unraveling the neuroprotective potential of scalp electroacupuncture in ischemic stroke: A key role for electrical stimulation.

This study investigates the therapeutic effects and mechanisms of electroacupuncture (EA) on cognitive impairment induced by chronic hypoxia (CH) in mice. Chronic hypoxia was simulated by exposing mice to a 10 % oxygen environment for 8 hours daily over 3 months. The cognitive functions of the mice were assessed through behavioral tests, including the open field test (OFT), Y-maze, and Morris water maze (MWM). Results showed that CH induced significant anxiety-like behaviors and memory impairments in mice. EA treatment, targeting the Baihui (GV20), Shenting (GV24), and Zusanli (ST36) acupoints, significantly ameliorated these behavioral deficits. Histological analyses using HE staining, Nissl staining, and TUNEL assays demonstrated that EA reduced neuronal damage, apoptosis, and myelin loss in the cerebral cortex and hippocampus. Additionally, EA treatment significantly lowered the expression of the pro-inflammatory cytokine TNF-α in brain tissues, suggesting its anti-inflammatory effects. Immunofluorescence and Western blot analyses revealed that EA inhibited the overactivation of microglia and astrocytes in the CH model. Specifically, EA suppressed the expression of Iba1 and GFAP, markers of microglial and astrocytic activation, respectively. Furthermore, EA promoted the polarization of microglia towards the M2 anti-inflammatory phenotype by downregulating iNOS and upregulating Arg1. Similarly, EA reduced the expression of C3, a marker of A1 astrocytes, thereby preventing astrocytic polarization towards the pro-inflammatory state. Organotypic brain slice cultures subjected to oxygen-glucose deprivation (OGD) confirmed that electrical stimulation, akin to EA, inhibited the activation of microglia and astrocytes under hypoxic conditions. In conclusion, EA improves cognitive function in CH-induced mice by reducing neuroinflammation, inhibiting glial cell overactivation, and promoting anti-inflammatory phenotypes. These findings highlight EA's potential as a therapeutic intervention for cognitive impairments related to chronic hypoxia.

Electroacupuncture mitigates cognitive impairments in chronic hypoxia-induced mice by modulating neuroinflammation.

This study aimed to investigate the association between anion gap (AG) and clinical outcomes in patients with trauma-induced coagulopathy (TIC), and to evaluate the predictive value of dynamic AG trajectories for 28-day mortality.

Impact of anion gap on in-hospital mortality in trauma-induced coagulopathy patients: a MIMIC-IV study.

Renal dysfunction is common in ischemic ischemic ischemic stroke patients, but the prognostic value of dynamic creatinine trajectories remains unclear.

ArticleTotal : 2	Year
Publication title Cited by 1	2023
Publication title	2026

Article	Year
Scalp Electroacupuncture Promotes Angiogenesis after Stroke in Rats by Activation of Wnt/-Catenin Signal Pathway. Evidence-based complementary and alternative medicine : eCAM\| PubMed ID: 35321503	2022
Therapeutic Effects of Kangzhi Syrup in a Guinea Pig Model of Ovalbumin-Induced Cough Variant Asthma. Evidence-based complementary and alternative medicine : eCAM\| PubMed ID: 29951106	2018
Bufei Decoction Alleviated Bleomycin-Induced Idiopathic Pulmonary Fibrosis in Mice by Anti-Inflammation. Evidence-based complementary and alternative medicine : eCAM\| PubMed ID: 32963571	2020
Professor 's clinical experience in treatment of with acupuncture. Zhongguo zhen jiu = Chinese acupuncture & moxibustion\| PubMed ID: 38467506	2024
Unraveling the neuroprotective potential of scalp electroacupuncture in ischemic stroke: A key role for electrical stimulation. Neuroscience\| PubMed ID: 39401739	2024
Electroacupuncture mitigates cognitive impairments in chronic hypoxia-induced mice by modulating neuroinflammation. IBRO neuroscience reports\| PubMed ID: 40144798	2025
Impact of anion gap on in-hospital mortality in trauma-induced coagulopathy patients: a MIMIC-IV study. European journal of medical research\| PubMed ID: 41015766	2025
Prognostic value of early creatinine trajectories in ischemic stroke patients: Insights from latent growth mixture modeling using MIMIC-IV. Clinical neurology and neurosurgery\| PubMed ID: 41518936	2026

Pengyu Zhu

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