Dergi
Optimized CRISPR-Based Molecular Diagnostics Protocol for Point-of-Care Use
August 16, 2024
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Özet

Here, we present the step-by-step preparation of premixed, lyophilized recombinase-based isothermal amplification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based reactions, which can be used for the detection of nucleic acid biomarkers of infectious disease pathogens or other genetic markers of interest.