Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Layla Malouf; Diana A. Tanase; Lorenzo Di Michele

doi:10.3791/66738

Dergi

Biyomühendislik

Versatile Biomolecular Condensates from Amphiphilic DNA Nanostars or C-Stars

May 31, 2024

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

01:19 min

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Cleaning of Glass Capillaries for the Preparation of C-Star Condensates

01:37 min

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Single-Component Condensate Formation from C-Star Mixtures in Glass Capillary Tubes

01:50 min

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Extraction of C-Star Single-Component Condensates from Glass Capillary Tubes

01:25 min

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Transcription of an RNA Aptamer from RNA-Templating C-Star Condensates

01:59 min

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Layla Malouf^1,2, Diana A. Tanase^1,2, Lorenzo Di Michele^1,2,3

¹Department of Chemical Engineering and Biotechnology,University of Cambridge, ²Kimya Bölümü,Imperial College London, ³fabriCELL,Imperial College London

Özet

We present a protocol for preparing synthetic biomolecular condensates consisting of amphiphilic DNA nanostars starting from their constituent DNA oligonucleotides. Condensates are produced from either a single nanostar component or two components and are modified to sustain in vitro transcription of RNA from an embedded DNA template.