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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

31,288 Views

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13:10 min

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September 08, 2010

DOI:

10.3791/2002-v

DOI:
10.3791/2002-v

•

13:10 min
September 08, 2010

•

6 Views
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1Biyoloji Bölümü,University of Rochester

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This article describes GFP-based fluorescence in vivo assays that separately quantify homologous recombination and nonhomologous end joining in mammalian cells.

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DNA Double-strand BreakRepairMammalian CellsNonhomologous End JoiningNHEJHomologous RecombinationHRPerturbationsPremature AgingTumorigenesisQuantitative MeasurementFluorescent Reporter ConstructsGFP GeneI-SceI EndonucleaseInduction Of DSBsGFP Positive CellsFlow Cytometry

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Seluanov, A., Mao, Z., Gorbunova, V. Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells. J. Vis. Exp. (43), e2002, doi:10.3791/2002 (2010).

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