Extraction of C-Star Single-Component Condensates from Glass Capillary Tubes

To begin, prepare a large microcentrifuge tube containing 60 microliters of 0.3 molar sodium chloride in tris EDTA buffer. Then unwrap the previously prepared capillary sample glued to the cover slip, and use a diamond scribing pen to score the underside of the cover slip at each inner end of the glued area. Break off the cover slip in this region and discard it appropriately.

Thoroughly clean the capillary tube with ethanol before drying it. Next, score each end of the capillary tube with the diamond scribing pen, first on the underside, then flipping and scoring the tube right side up. Ensure the score lines do not overlap the oil-water interface.

Snap off the ends of the capillary tubes, retaining the central part and discarding the rest. Place the cut capillary tube into the previously prepared microcentrifuge tube, ensuring the bottom of the capillary tube makes contact with the buffer solution. Allow the condensates to sediment from the tube into the buffer reservoir under gravity for a minimum of 10 minutes.

Finally, remove the cut capillary tube and discard it appropriately.