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Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
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May 25, 2019
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In vitro fertilization is a commonly used technique with a variety of model organisms to maintain lab populations and produce synchronized embryos for downstream applications. Here, we present a protocol that implements this technique for different populations of the Mexican tetra fish, Astyanax mexicanus.
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Peuß, R., Zakibe, Z., Krishnan, J., Merryman, M. S., Baumann, D. P., Rohner, N. Gamete Collection and In Vitro Fertilization of Astyanax mexicanus. J. Vis. Exp. (147), e59334, doi:10.3791/59334 (2019).
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