Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Laurel R. Yohe; Paolo Devanna; Kalina  T.J. Davies; Joshua H.T. Potter; Stephen  J. Rossiter; Emma  C. Teeling; Sonja C. Vernes; Liliana  M. D&#225;valos

doi:10.3791/59505

-Omics Analizleri ve Birincil Hücre Kültürü için Yarasaların Doku Toplama

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Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

-Omics Analizleri ve Birincil Hücre Kültürü için Yarasaların Doku Toplama

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15:31 min

October 23, 2019

DOI: 10.3791/59505-v

Laurel R. Yohe*^1,2, Paolo Devanna*³, Kalina T.J. Davies⁴, Joshua H.T. Potter⁴, Stephen J. Rossiter⁴, Emma C. Teeling⁵, Sonja C. Vernes*^3,6, Liliana M. Dávalos*^2,7

¹Department of Geology & Geophysics,Yale University, ²Department of Ecology & Evolution,Stony Brook University, ³Neurogenetics of Vocal Communication,Max Planck Institute for Psycholinguistics, ⁴School of Biological and Chemical Sciences,Queen Mary University of London, ⁵School of Biology & Environmental Science,University College Dublin, ⁶Donders Institute for Brain, Cognition and Behavior, ⁷Consortium for Inter-Disciplinary Environmental Research,Stony Brook University

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Overview

The study presents a protocol for optimal tissue preparation essential for genomic, transcriptomic, and proteomic analyses of wild-caught bats. It emphasizes humane bat capture and meticulous dissection to maximize the quality of collected molecular data while minimizing impact on bat populations.

Key Study Components

Research Area

Genomic analyses
Transcriptomic studies
Proteomic profiling

Background

Importance of tissue preservation in molecular biology
Non-lethal sampling methods for bats
Advancements in techniques for tissue collection and storage

Methods Used

Step-by-step humane capture of bats
Dissection and preservation techniques for various tissues
Use of RNA stabilizing solutions for molecular preservation

Main Results

Detailed protocols for dissection that enhance the quality of collected data
Successful preservation methods for multiple tissue types ensuring their viability for future studies
Generated sufficient material for both genomic and functional studies without lethal sampling

Conclusions

Underscores the necessity of non-lethal methods for sampling wild animals
Provides a framework for effective molecular studies in bat populations, crucial for ecological research

Frequently Asked Questions

What is the main goal of this tissue preparation protocol?

The protocol aims to optimize tissue collection for downstream genomic, transcriptomic, and proteomic analyses.

Why is humane capture important in this research?

Humane capture minimizes the impact on bat populations while allowing for valuable scientific data collection.

What types of tissues can be collected using this protocol?

Various tissues including brain, muscle, lung, heart, liver, and reproductive organs can be collected.

How are tissues preserved after collection?

Tissues are preserved in RNA stabilizing solutions or flash-frozen in liquid nitrogen for future analysis.

What is the advantage of using non-lethal sampling methods?

Non-lethal methods allow for ongoing studies of the same populations without depleting their numbers.

Can this protocol be applied to other species?

While this protocol is tailored for bats, similar principles can be adapted for other wildlife studies.

What technologies are essential for this protocol?

Essential technologies include RNA stabilization solutions and methods for preserving high molecular weight DNA.

Bu, vahşi doğada yakalanan yarasaların genomik, transkripsiyonik ve proteomik analizleri için en uygun doku hazırlığı için bir protokoldür. Bu yarasa yakalama ve diseksiyon, doku koruma ve yarasa dokusunun hücre culturing için protokoller içerir.

Bu videoda, popülasyonlar üzerindeki etkiyi en aza indirmek ve bilimsel değeri en üst düzeye çıkarmak amacıyla yarasaları insancıl bir şekilde toplamak için adım adım kılavuz salıyoruz. Yaklaşımımızın avantajları, her yarasa için potansiyel moleküler ve morfolojik veri miktarını en üst düzeye çıkarmak, gelecekteki değerlerini korumak için dokuları korumak ve yabani bireylerin hasat en aza indirmek tir. Diseksiyon protokollerini sözlü olarak göstermek zordur.

Burada sunduğumuz doku hazırlama tekniklerinin çoğu, farklı organların nasıl tanımlandığını görselleştirerek en iyi şekilde iletilir ve daha sonra düzgün bir şekilde incelenir ve depolanır. Gecikmeleri önlemek için herhangi bir diseksiyon başlamadan önce tüm şişeleri hazırlayın. RNA için, her numune için gerekli şişe sayısını bir kenara koyun.

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