Jinekolojik ve Meme Kanseri Tümörlerinden Kanser Kök Hücre Kürelerinin Alınması

9.6K görüntüleme

7 kez atıf

07:01 dk

1 Mart 2020

10.3791/60022-v

1 Mart 2020

9.6K görüntüleme

Mafalda Laranjo^*¹^,²^,³^,⁴ , Maria João Carvalho^*¹^,²^,³^,⁴^,⁵^,⁶ , Beatriz Serambeque¹^,²^,³ , André Alves²^,⁷ , Carlos Miguel Marto¹^,²^,³^,⁴^,⁸ , Isabel Silva⁹ , Artur Paiva³^,⁹^,¹⁰ , Maria Filomena Botelho¹^,²^,³^,⁴^,⁸

¹Institute of Biophysics, Faculty of Medicine, University of Coimbra, ²Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, ³CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ⁴Clinical Academic Center of Coimbra (CACC), ⁵Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, ⁶Gynecology A Service, Coimbra Hospital and Universitary Center, ⁷Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, ⁸Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, ⁹Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, ¹⁰Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences

^* These authors contributed equally

Bu metodolojinin amacı, kanser hücre hatlarındaki kanser kök hücrelerini (CSC) ve primer insan tümör örneklerini küre oluşturma protokolü ile sağlam bir şekilde, fonksiyonel tahliller ve akış sitometrisi ve Batı ile henotypik karakterizasyon kullanarak tanımlamaktır. Leke.