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Dissection of Saccharomyces Cerevisiae Asci
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Dissection of Saccharomyces Cerevisiae Asci

Article DOI: 10.3791/1146-v • 12:58 min • May 19th, 2009
May 19th, 2009 •
1, 1, 1
1Department of Biology, Concordia University

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Micromanipulation of yeast cells is needed for meiotic genetic analysis or to select diploid zygotes. These micromanipulations are carried out using the microneedle of a dissection microscope. The microneedle is used to relocate cells and is controlled by a micromanipulator which are available with various degrees of automation.

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Saccharomyces Cerevisiae Yeast Model System Cellular Processes Haploid Diploid Alleles Genome Modifications Asci Production Mating Types Diploid Strain Solid Medium Liquid Medium Growth Selection Sporulation Nutrient-poor Medium Meiotic Reproduction Ascospores Zymolyase Enzyme Membrane Sac Micromanipulation Dissection Microscope Ascopores Separation Replica Plating Selective Media
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