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Visualizing Antigen-spezifischen CD4 + T-Zellen mit MHC Klasse II Tetramere
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Visualizing Antigen-spezifischen CD4 + T-Zellen mit MHC Klasse II Tetramere

Article DOI: 10.3791/1167-v • 15:43 min • March 6th, 2009
March 6th, 2009 •
1, 2, 3, 2
1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute

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Dieses Verfahren zeigt die Reinigung und in vitro Expansion von Antigen-spezifischen CD4 + T-Zellen aus ganzen peripheren Blut und deren Visualisierung mit Hilfe der MHC-Klasse II Tetramere. Tetramere erlauben die direkte Visualisierung von T-Zellen mit einem einzigen Antigen-Spezifität und definierte MHC-Klasse II Einschränkung.

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Immunologie CD4 + T-Zellen MHC-Klasse II Tetramere peripheren mononukleären Zellen in vitro Expansion Durchflusszytometrie
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