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JoVE Journal
Biology

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一种高通量的方法,在全球范围内研究在酿酒酵母中的细胞器形态
 
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一种高通量的方法,在全球范围内研究在酿酒酵母中的细胞器形态

Article DOI: 10.3791/1224-v 07:29 min March 2nd, 2009
March 2nd, 2009

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Summary

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Click here for the English version.

GFP融合蛋白被广泛应用于共聚焦显微镜可视化的细胞器。然而,筛选突变,影响细胞器的形态一般需要个人的突变,非常耗时。在这里,我们展示了一种方法,同时结合近5000个非必要基因在酵母细胞器- GFP标记。

Tags

微生物学,ACP1,共聚焦显微镜,高通量的第25期,线粒体,内质网,酿酒酵母
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