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一种高通量的方法,在全球范围内研究在酿酒酵母中的细胞器形态
JoVE Journal
Biology
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JoVE Journal Biology
A high-throughput method to globally study the organelle morphology in S. cerevisiae
DOI:

07:29 min

March 02, 2009

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Chapters

  • 00:00Title
  • 01:00Introduction
  • 01:47Preparation for Incorporating Acp1- GFP in the DMA
  • 03:16Mating and Diploid Selection
  • 03:50Sporulation and Germination
  • 04:48Selection of Both Alleles
  • 05:28Microscopy
  • 06:02Representative Results/ Outcome
  • 06:28Conclusion

Summary

Automatic Translation

GFP融合蛋白被广泛应用于共聚焦显微镜可视化的细胞器。然而,筛选突变,影响细胞器的形态一般需要个人的突变,非常耗时。在这里,我们展示了一种方法,同时结合近5000个非必要基因在酵母细胞器- GFP标记。

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