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Zebrafisch Whole Mount High-Resolution Doppel Fluorescent In-situ Hybridisierung
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Zebrafisch Whole Mount High-Resolution Doppel Fluorescent In-situ Hybridisierung

Article DOI: 10.3791/1229-v • 12:31 min • March 25th, 2009
March 25th, 2009 •
1, 1
1Department of Molecular, Cellular and Developmental Biology, Yale University

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Whole mount in situ Hybridisierung ist eine der am häufigsten verwendeten Techniken in der Entwicklungsbiologie. Hier präsentieren wir ein hochauflösendes Doppel-Fluoreszenz-in situ-Hybridisierung Protokoll zur Analyse der genauen Expressionsmuster eines einzelnen Gens und zur Bestimmung der Überlappung der Ausdruck Domains von zwei Genen. Wir gehören eine Propidiumiodid nukleare Gegenfärbung des Gewebes Organisation hervorzuheben.

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