34,496 Views
•
07:03 min
•
July 02, 2009
DOI:
Summary
Automatically generated
Dissociar as células de tipos de tecido específico requer parâmetros específicos para a agitação do tecido para obter um volume elevado de viáveis, as células cultiváveis. A Miltenyi gentleMACS dissociator otimiza essa tarefa com um protocolo simples e prática. Nesta publicação o uso deste aparelho no tecido pulmonar é explicado.
Related Videos
09:04
Isolation of Mouse Lung Dendritic Cells
Related Videos
28992 Views
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Related Videos
37665 Views
08:46
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Related Videos
11772 Views
11:17
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Related Videos
22451 Views
16:55
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
Related Videos
20373 Views
09:15
Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers
Related Videos
857 Views
06:02
Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung
Related Videos
452 Views
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
Related Videos
8292 Views
04:03
Mouse Wound Models and Preparation of Single-Cell Suspensions
Related Videos
418 Views
07:55
Isolation of Lung Retinoid-Containing Cells by Cell Sorting
Related Videos
161 Views
Read Article
Cite this Article
Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D., Bosio, A. Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator. J. Vis. Exp. (29), e1266, doi:10.3791/1266 (2009).
Copy