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07:03 min
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July 02, 2009
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Summary
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Диссоциирующего клетки определенных типов ткани требует специфических параметров для агитации ткани для получения большого количества жизнеспособных, culturable клеток. Miltenyi gentleMACS диссоциатор оптимизирует эту задачу с простым, практичным протокола. В этой публикации использование этого аппарата на легочной ткани объясняется.
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Cite this Article
Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D., Bosio, A. Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator. J. Vis. Exp. (29), e1266, doi:10.3791/1266 (2009).
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