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Chapters
- 00:05Title
- 01:08Introduction
- 01:43Preparation of Reagents
- 04:00The gentleMACS Tissue Dissociation
- 07:22Filtration
- 09:35Representative Results
- 10:25Conclusion
Dissociar as células de tipos de tecido específico requer parâmetros específicos para aggitation tecido para obter um volume elevado de viáveis, as células cultiváveis. A Miltenyi gentleMACS Dissociator otimiza essa tarefa com um protocolo simples e prática. Nesta publicação o uso deste aparelho no tecido nerual é explicado.
Tags
Single-cell SuspensionsNervous SystemNeuronal Cell TypesGlial Cell TypesCell Surface MoleculesMolecular DeterminantsCell IdentificationCell Separation TechnologiesGentleMACS DissociatorTrypsin-based Dissociation KitsPapain-based Dissociation KitsAntigen EpitopesCell LossC TubesGentleMACS ProgramsMonoclonal ConjugatesAnti-Prominin-1 MicroBeadsNeural ProgenitorsMyelin Removal Beads
