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Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
 
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Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Article DOI: 10.3791/1274-v 10:05 min August 12th, 2009
August 12th, 2009

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Summary

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Mating and tetrad separation are required for genetic analysis in Chlamydomonas reinhardtii. Here we demonstrate standard methods for gametogenesis, mating, zygote germination and tetrad dissection. This protocol consists of an easy-to-follow series of steps that will make genetic approaches amenable to scientists who are less familiar with Chlamydomonas.

Tags

Mating Tetrad Separation Chlamydomonas Reinhardtii Genetic Analysis Cell Biology Genetics Life Cycle Growth Manipulation Genomic Resources Transformability Nucleus Organelles Vegetative Diploids Dominance Analysis Tetrad Dissection Nuclear Inheritance Organellar Inheritance Allelism Test Epistasy Analysis Map-based Cloning Gametogenesis Zygote Germination
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