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Journal / Biology / Muis bijnier Chromaffinecellen Cell Isolatie
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Muis bijnier Chromaffinecellen Cell Isolatie
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Muis bijnier Chromaffinecellen Cell Isolatie

Article DOI: 10.3791/129 • 18:30 min
January 5th, 2007 •
1, 2,3, 4, 1, 2,3
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Physiology and Biophysics, University of Southern California, Keck School of Medicine, 3Zilkha Neurogenetic Institute, University of Southern California, Keck School of Medicine, 4Department of Developmental and Cell Biology, University of California, Irvine (UCI)

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January 5th, 2007

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Bijniermerg chromaffin celkweek systemen zijn uiterst nuttig voor de studie van de excitatie-secretie koppeling in een in vitro setting. Dit protocol geeft de methode die wordt gebruikt om de bijnieren ontleden en vervolgens de medullaire regio te isoleren door het strippen van weg de bijnierschors. De vertering van de medulla in enkele chroomaffiene cellen wordt dan aangetoond.

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