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Journal / Biology / Protein Ubikitinasyon Tespiti
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JoVE Journal Biology
Protein Ubikitinasyon Tespiti
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Journal (Biology)
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
Journal (Biology)
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
 
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Protein Ubikitinasyon Tespiti

Article DOI: 10.3791/1293-v • 09:00 min • August 19th, 2009
August 19th, 2009 •
1, 1
1Signal Transduction Program, The Sanford Burnham Institute for Medical Research

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Ubikitinasyon üç enzimlerin kümesi tarafından yürütülen bir anahtar posttranslasyonel modifikasyonu. Bu değişiklik ilgili genler Mutasyonlar birçok farklı insan hastalıkları ile ilişkilidir. Burada, kültür hücrelerinde protein ubikuitinasyon tespit protokolleri tarif

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Hücre Biyoloji Biyokimya Sayı 30 in vitro sistemi immunoprecipitation ubikuitinasyon kültürlü hücre, immunoblotting ubikuitin posttranslasyonel modifikasyonu
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