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JoVE Journal
Biology

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Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
 
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Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

Article DOI: 10.3791/134-v 15:07 min January 30th, 2007
January 30th, 2007

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Summary

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Click here for the English version.

This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.

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Growth Factor-coated Bead Placement Dorsal Forebrain Explants
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