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Biology

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JoVE Journal Biology
Un metodo HPLC lectina per arricchire Peptidi selettivamente glicosilata da campioni biologici complessi
 
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Un metodo HPLC lectina per arricchire Peptidi selettivamente glicosilata da campioni biologici complessi

Article doi: 10.3791/1398
October 1st, 2009 •
1, 2, 1, 1, 1, 2, 1, 1, 1, 3, 2, 1, 1
1Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco - UCSF, 2Buck Institute for Age Research, 3Department of Chemistry, Purdue University

Summary
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October 1st, 2009

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Lectina coniugato perline POROS sono stati impiegati per HPLC. Standard glicopeptide serviti come controlli positivi e negativi. MARS-14 impoverito, tripsina-digerito plasmatiche umane è stato cromatografato e flow-through (FT) e le frazioni legato raccolti per analisi ESI-LC-MS/MS. Glicopeptidi sono stati arricchiti nella frazione legata, rispetto a FT.

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