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Obtention d'ARN de haute qualité provenant des populations cellule unique de tissus post-mortem du cerveau de l'homme
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Obtention d'ARN de haute qualité provenant des populations cellule unique de tissus post-mortem du cerveau de l'homme

Article DOI: 10.3791/1444-v • 18:17 min • August 6th, 2009
August 6th, 2009 •
1, 1, 1,2,3
1Department of Structural and Molecular Neuroscience, McLean Hospital, 2Department of Psychiatry, Harvard Medical School, 3Department of Psychiatry, Beth Israel Deaconess Medical Center

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Nous décrivons un processus utilisant le laser capture microdissection d'isoler et d'extraire l'ARN à partir d'une population cellulaire homogène, les neurones pyramidaux, dans la couche III du gyrus temporal supérieur dans les cerveaux post-mortem humain. Nous avons ensuite linéairement amplifier (T7-based) ARNm, et s'hybrident l'échantillon à la puce Affymetrix humaines X3P.

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Neurosciences No. 30 autopsie les microréseaux l'ARN gyrus temporal supérieur laser capture microdissection les neurones pyramidaux
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