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Journal / Biology / İnsan Postmortem Beyin Dokusu Tek Hücre…
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İnsan Postmortem Beyin Dokusu Tek Hücre Popülasyonlar Yüksek Kalite RNA Alınması
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İnsan Postmortem Beyin Dokusu Tek Hücre Popülasyonlar Yüksek Kalite RNA Alınması

Article DOI: 10.3791/1444-v • 18:17 min • August 6th, 2009
August 6th, 2009 •
1, 1, 1,2,3
1Department of Structural and Molecular Neuroscience, McLean Hospital, 2Department of Psychiatry, Harvard Medical School, 3Department of Psychiatry, Beth Israel Deaconess Medical Center

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Biz postmortem insan beyni superior temporal girus III. Tabakası homojen bir hücre popülasyonu, piramidal nöronlar, RNA izole etmek ve ayıklamak için lazer yakalama mikrodiseksiyon kullanarak bir süreci tanımlar. Biz (T7 tabanlı) mRNA daha sonra doğrusal yükseltmek. Kullanmakta olduğunuz örnek Affymetrix insan X3P mikroarray melezleşme.

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Nörobilim Sayı 30 Postmortem mikroarray'ler RNA superior temporal girus lazer yakalama mikrodiseksiyon piramidal nöronlar
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