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Journal / Biology / Produção lentivírus
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JoVE Journal Biology
Produção lentivírus
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Journal (Biology)
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids (Video) | JoVE
Journal (Biology)
Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids (Video) | JoVE
 
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Produção lentivírus

Article DOI: 10.3791/1499-v • 11:43 min • October 2nd, 2009
October 2nd, 2009 •
1, 1
1Diabetes Center, University of California, San Francisco - UCSF

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Para fazer lentivírus, vetores de DNA são transfectadas em células humanas 293. Após a colheita e concentrando-se o sobrenadante, título de vírus é determinado pela expressão de fluorescência com um citômetro de fluxo.

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Microbiologia de Emissão 32 Lentivirus RNAi titulação viral transfecção retrovírus citometria de fluxo sistema de vetor split shRNA.
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