Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation and Large Scale Expansion of Adult…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive
Journal (Biology)
Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
Journal (Biology)
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
 

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Article DOI: 10.3791/1524-v • 12:18 min • October 28th, 2009
October 28th, 2009 •
1, 1, 1
1Stem Cell Research Unit, Medical University of Graz, Austria

Chapters

Summary
Automatic Translation

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

Tags

Isolation Large Scale Expansion Adult Human Endothelial Colony Forming Progenitor Cells Recovery Strategy Heparinized Blood Peripheral Blood PHPL Bovine Serum Antigens Flow Cytometry Immunohistochemistry In Vitro Functionality Vascular Structures Matrigel Assay Subcutaneous Injection Mouse Model Perfused Vessels Cryopreservation Proliferation Genomic Stability Animal-protein Free Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter