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JoVE Journal
Biology
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Chick ex ovo Culture and ex ovo CAM Assay
 
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Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Article DOI: 10.3791/1620-v 17:23 min November 30th, 2009
November 30th, 2009

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Summary

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Click here for the English version.

The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.

Tags

Chick Ex Ovo Culture Ex Ovo CAM Assay Chicken Eggs Angiogenesis Tumorigenesis Chick Chorioallantoic Membrane CAM Blood Vessel Network Immunodeficient Host Grafting Tissues And Cells Tumor Cell Intravasation Micro Metastatic Foci Experimental Limitations Shell-less Cultures Survival Rates
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