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Derivazione di cellule staminali ematopoietiche da cellule staminali embrionali murini
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Derivazione di cellule staminali ematopoietiche da cellule staminali embrionali murini

Article DOI: 10.3791/162-v • 22:07 min • February 25th, 2007
February 25th, 2007 •
1, 1
1Childrens Hospital, Harvard Stem Cell Institute, Harvard Medical School

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Questa dettagli del protocollo la derivazione di cellule staminali ematopoietiche trapiantabili da topo cellule staminali embrionali (ESC) e la loro successiva iniezione in topi irradiati letalmente destinatario. In breve, ESC si differenziano come corpi embrionali, che sono poi infettati con HoxB4 retrovirali e co-coltura con cellule stromali OP9 e citochine ematopoietiche.

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Biologia Cellulare cellule staminali cellule staminali HSC i trapianti HoxB4
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