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Mess-Diffusionskoeffizienten über Zwei-Photonen-Fluorescence Recovery After Photobleaching
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Mess-Diffusionskoeffizienten über Zwei-Photonen-Fluorescence Recovery After Photobleaching

Article DOI: 10.3791/1636-v • 07:00 min • February 26th, 2010
February 26th, 2010 •
1, 2
1Department of Physics and Astronomy, University of Rochester, 2Department of Biomedical Engineering, University of Rochester

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In diesem Artikel beschreiben wir das Verfahren zur Messung von Diffusionskoeffizienten mittels Multi-Photonen-Fluoreszenz-Erholung nach Ausbleichen. Wir werden durch die Angleichung der Laser entlang der optischen Pfad zu der Probe und Bestimmung der richtigen experimentellen Parameter beginnen, dann weiter generiert und schließlich passende Fluoreszenz Erholung Kurven.

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Cellular Biology Ausgabe 36 Diffusion Fluorescence Recovery After Photobleaching MP-FRAP FPR Multi-Photonen-
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