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Misurare coefficienti di diffusione via a due fotoni di recupero fluorescenza Dopo photobleaching
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Misurare coefficienti di diffusione via a due fotoni di recupero fluorescenza Dopo photobleaching

Article DOI: 10.3791/1636-v • 07:00 min • February 26th, 2010
February 26th, 2010 •
1, 2
1Department of Physics and Astronomy, University of Rochester, 2Department of Biomedical Engineering, University of Rochester

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In questo articolo andremo a descrivere il procedimento per determinare coefficienti di diffusione multi-fotone con il recupero di fluorescenza dopo photobleaching. Inizieremo allineando il laser lungo il percorso ottico al campione e determinare i parametri corretti sperimentale, poi continuare a generare ed infine montaggio curve recupero fluorescenza.

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Biologia Cellulare Numero 36 diffusione il recupero di fluorescenza dopo photobleaching MP-FRAP FPR multi-fotone
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