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January 04, 2010
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يمكن أن نقاط الاشتباك العصبي Glutamatergic التبديل من الوضع النشط لوضع صامت. علينا أن نظهر أن حالة النشاط قبل المشبكي في الثقافة فصلها من الخلايا العصبية القوارض وتصور باستخدام نموذج قابل للتثبيت الصبغة FM1 - 43 لتصور نقاط الاشتباك العصبي نشطة وimmunostaining مع vGluT 1 - الضد لتصور كل نقاط الاشتباك العصبي الغلوتامات.
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Cite this Article
Moulder, K. L., Jiang, X., Taylor, A. A., Benz, A. M., Mennerick, S. Presynaptically Silent Synapses Studied with Light Microscopy. J. Vis. Exp. (35), e1676, doi:10.3791/1676 (2010).
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