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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Article DOI: 10.3791/1698-v • 14:04 min • February 1st, 2010
February 1st, 2010 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Biochemistry, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto

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MYTH allows the sensitive detection of transient and stable interactions between proteins that are expressed in the model organism Saccharomyces cerevisiae. It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner.

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Split-ubiquitin Membrane Yeast Two-hybrid Protein-protein Interactions MYTH System Full-length Transmembrane Proteins Saccharomyces Cerevisiae Bait Protein Prey Proteins Ubiquitin Moieties Artificial Transcription Factor Pseudo-ubiquitin Molecule Deubiquitinating Enzymes Reporter Gene Expression
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