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Split-Ubiquitine membraanfilters op Yeast Two-Hybrid (mythe) Systeem
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Split-Ubiquitine membraanfilters op Yeast Two-Hybrid (mythe) Systeem: een krachtig hulpmiddel voor het identificeren van eiwit-eiwit interacties

Article DOI: 10.3791/1698-v • 14:04 min • February 1st, 2010
February 1st, 2010 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Biochemistry, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto

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MYTHE kan de gevoelige detectie van voorbijgaande aard en stabiele interacties tussen eiwitten die worden uitgedrukt in het model organisme Saccharomyces cerevisiae. Het is met succes toegepast op exogene en gist integrale membraaneiwitten bestuderen om hun interagerende partners in een high throughput manier te identificeren.

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Cellular Biology eiwit-eiwit interacties membraan split-ubiquitine gist bibliotheek screening Y2H gist twee-hybride MYTHE
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