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Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

Article DOI: 10.3791/1733-v • 11:26 min • May 5th, 2010
May 5th, 2010 •
1, 1,2, 1
1Department of Horticulture and Crop Science, The Ohio State University, 2Department of Plant Pathology, North Carolina State University

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Summary
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We report a method for introduction, tracking and quantitative analysis of GFP expression in plant cells. This method utilizes a custom-designed robotics system for semi-continuous image collection from large numbers of samples, over time. We also demonstrate the use of ImageJ and ImageReady for analysis of image series.

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Robotics Dynamic Image Analysis Gene Expression Plant Tissues Green Fluorescent Protein (gfp) Promoters Lima Bean Cotyledonary Tissues Particle Bombardment Robotic Image Collection System Fluorescence Dissecting Microscope Digital Camera Robotics Platform Culture Dishes Expression Profiles Manual Image Alignment ImageReady Software GFP Intensity Measurements ImageJ Software Background Fluorescence
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