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Article doi: 10.3791/1746
March 17th, 2010

Summary March 17th, 2010

Please note that all translations are automatically generated.

Click here for the English version.

我们采用无标记蛋白质相互作用分析利用Biacore X100几个胱抑素乙突变体的结合,木瓜蛋白酶,通过动力学特性的结构与功能分析。无标定浓度分析中心(CFCA)的措施,而不需要为标准曲线与保留结合活性的蛋白浓度。我们表明,浓度使用CFCA增加的动力学分析的可靠性,并能够可靠地确定,即​​使重组蛋白的活性降低,动力学常数确认。

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