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JoVE Journal
Biology

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分离和富集大鼠间质干细胞(MSCS)及衍生的干细胞分离单细胞集落
 
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分离和富集大鼠间质干细胞(MSCS)及衍生的干细胞分离单细胞集落

Article DOI: 10.3791/1852-v 08:19 min March 22nd, 2010
March 22nd, 2010

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

大鼠MSCs从股骨和胫骨分离,然后通过磁性细胞分选富集。排序细胞被证实为流式细胞仪检测表面标志物表达。这些细胞克隆密度培养,形成单菌落,然后这些菌落克隆气瓶分开。

Tags

细胞生物学,第37期,间质干细胞,磁性细胞分选,流式细胞仪,克隆缸
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