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Testen der Fähigkeit des diffusiblen Signalmoleküle zu embryonalen Spinal kommissuralen Axone neu ausrichten
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Testen der Fähigkeit des diffusiblen Signalmoleküle zu embryonalen Spinal kommissuralen Axone neu ausrichten

Article DOI: 10.3791/1853-v • 09:29 min • March 8th, 2010
March 8th, 2010 •
*1,2, *1, *1,2, 1,2
1Department of Biological Sciences, University of Southern California, 2Neuroscience Graduate Program, University of Southern California
* These authors contributed equally

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Dieser Test bewertet die Fähigkeit eines Signalmolekül, hier Bone Morphogenetic Protein 7 (BMP7), um kommissuralen Axone neu zu orientieren. Eine Explantation von embryonalen Rückenmark kultiviert wird neben einem Aggregat von COS-Zellen sekretieren der Kandidat Wachstumsfaktoren. Umorientiert kommissuralen Axone wachsen innerhalb der Explantation werden visualisiert durch Immunhistochemie.

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Neuroscience Ausgabe 37 kommissuralen Axone Rückenmark Ratte Explantation Kollagen COS-Zellen Knochen Proteine ​​(BMPs)
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