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Die Anwendung der Stopped-Flow-Kinetics Methoden der Wirkmechanismus eines DNA-Repair Protein Untersuchen
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Die Anwendung der Stopped-Flow-Kinetics Methoden der Wirkmechanismus eines DNA-Repair Protein Untersuchen

Article DOI: 10.3791/1874-v • 11:01 min • March 31st, 2010
March 31st, 2010 •
1, 1, 1, 1
1Molecular Biology and Biochemistry Department, Wesleyan University

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MSH2-MSH6 ist verantwortlich für die Initiierung Reparatur der Replikation Fehler bei der DNA. Hier präsentieren wir eine transiente Kinetik Ansatz zum Verständnis, wie diese kritische Protein funktioniert. Der Bericht zeigt, stopped-flow Experimente zur Messung der gekoppelten DNA-Bindung und ATPase-Kinetik zugrunde liegenden MSH2-MSH6 Wirkmechanismus in der DNA-Reparatur.

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Cellular Biology Ausgabe 37 DNA-Mismatch-Reparatur Stopped-Flow-Kinetik MSH2-MSH6 ATPase-Rate DNA-Bindung
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