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Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
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Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Article DOI: 10.3791/1876-v • 07:39 min • May 17th, 2010
May 17th, 2010 •
1, 1
1Division of Chemical Biology, New England Biolabs

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SNAP-tag and CLIP-tag protein labeling systems enable the specific, covalent attachment of molecules, including fluorescent dyes, to a protein of interest in live cells. Once cloned and expressed, the tagged protein can be used with a variety of substrates for numerous downstream applications without having to clone again.

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Fluorescent Labeling COS-7 SNAP-tag Fusion Proteins Live Cell Imaging Protein Labeling Systems Covalent Attachment Fluorescent Dyes Protein Of Interest Imaging Instrumentation Cloning Expression Downstream Applications SNAP-tag Substrate Human O6-alkylguanine-DNA-alkyltransferase (hAGT) DNA Repair Protein Benzyl Linker Labeling Reaction CLIP-tag Orthogonal Labeling
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