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Pulse-chase Analisi di N-linked catene di zuccheri da glicoproteine ​​in cellule di mammifero
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Pulse-chase Analisi di N-linked catene di zuccheri da glicoproteine ​​in cellule di mammifero

Article DOI: 10.3791/1899-v • 10:18 min • April 27th, 2010
April 27th, 2010 •
1, 1, 1, 1, 1
1Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University

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Descriviamo un metodo per l'analisi dell'alterazione di N-glicani legati attraverso la vita inizi di glicoproteine ​​dopo la loro biosintesi in cellule di mammifero. Ciò si ottiene pulse-chase analisi di metabolicamente glicani etichettati, rilascio enzimatico da glicoproteine ​​e l'esame mediante HPLC.

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Biologia Cellulare Numero 38 N-linked oligosaccaridi mannosidasi mannosio-etichettatura la degradazione reticolo endoplasmatico associati calnexin glicosilazione,
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