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Journal / Biology / Entwicklung von automatisierten Imaging und…
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Entwicklung von automatisierten Imaging und Analyse für Zebrafisch chemische Bildschirme.
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Entwicklung von automatisierten Imaging und Analyse für Zebrafisch chemische Bildschirme.

Article DOI: 10.3791/1900-v • 10:50 min • June 24th, 2010
June 24th, 2010 •
1, 2, 3,4, 2, 2
1Pharmacology and Chemical Biology, University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics, University of Pittsburgh, 3Department of Pharmaceutical Sciences, University of Pittsburgh, 4Department of Chemistry, University of Pittsburgh

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Wir berichten über die Entwicklung eines Systems zur automatisierten Abbildung und Analyse von Zebrafisch-transgenen Embryos in Mikrotiterplatten. Dies demonstriert die Fähigkeit, dosisabhängige Wirkung eines kleinen Moleküls, BCI, auf Fibroblast Growth Factor Expression des Reportergens zu messen und Technologie für den Aufbau High-Throughput-Zebrafisch chemische Bildschirme.

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Cellular Biology Ausgabe 40 Zebrafisch Chemical Screens Cognition Network Technology Fibroblast Growth Factor (E)-2-Benzyliden-3-(cyclohexylamino) -2,3-dihydro-1H-inden-1-one (BCI) Tg (dusp6: d2EGFP)
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