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JoVE Journal Neuroscience
SDS-PAGE/Immunoblot Detectie van Aß multimeren in Human Corticale weefselhomogenaten met behulp van antigeen-epitoop
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SDS-PAGE/Immunoblot Detectie van Aß multimeren in Human Corticale weefselhomogenaten met behulp van antigeen-epitoop

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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Beschrijven we een techniek voor de bereiding van menselijke verduidelijkt corticale homogenaten, eiwit scheiding door SDS-PAGE, antigen retrieval en immunoblotting met een antilichaam tegen het Aß peptide. Met behulp van dit protocol, wij consequent te sporen monomere en multimere Aß in corticale weefsel van mensen met de ziekte van Alzheimer pathologie.

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Jove Neuroscience β-amyloïd oligomeren multimeren Western blotting aggregatie van eiwitten de ziekte van Alzheimer antigen retrieval
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