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Journal / Neuroscience / SDS-PAGE/Immunoblot Detektion von Aß Multimere in …
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Neuroscience

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JoVE Journal Neuroscience
SDS-PAGE/Immunoblot Detektion von Aß Multimere in Human Kortikale Gewebehomogenaten mit Antigen-Epitope Retrieval
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SDS-PAGE/Immunoblot Detektion von Aß Multimere in Human Kortikale Gewebehomogenaten mit Antigen-Epitope Retrieval

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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Wir beschreiben eine Technik für die Herstellung von geklärt menschlichen kortikalen Homogenate, Protein Trennung durch SDS-PAGE, Antigen-Retrieval und Immunoblot mit einem Antikörper gegen das Aß-Peptid. Mit diesem Protokoll, setzen wir konsequent erkennen monomeren und multimeren Aß in kortikalen Gewebe von Menschen mit Alzheimer-Pathologie.

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JoVE Neuroscience Ausgabe 38 β-Amyloid-Oligomere Multimere Western Blots Protein-Aggregation Alzheimer Antigen-Retrieval
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